2013年9月8日星期日

[ Fluorescent Brightener Fluorescent pig ]

Cloned transgenic pigs fluorescence experiment itself does not have practical value, but with a certain scientific value . Because green fluorescent protein marker role played cloned transgenic pigs fluorescence experiments showed somatic cell cloning technology  chrome oxide in pigs have been successful. If you put the green fluorescent protein gene ( or fusion on ) gene of practical value , such as human interferon resistance gene or genes , it is possible to get a resistance to disease or capable of producing human interferon transgenic cloned pigs . Cloned transgenic pigs fluorescence experiment itself does not have practical value, but with a certain scientific value . Because green fluorescent protein marker role played cloned transgenic pigs fluorescence experiments showed somatic cell cloning technology in pigs have been successful. Fluorescent pig test success , marking a state through somatic cell nuclear transfer technology to produce transgenic pigs  carbon black level of development. Between any organism can be genetically modified , and, in transgenic animals can also be genetically modified again . Some parts of the pig to fluoresce , is genetically result.
Reported that researchers first implanted in porcine somatic cells can synthesize the jellyfish green fluorescent protein gene , remove the nucleus after its implantation in pig unfertilized eggs , made of embryos, then implanted in the uterus of pigs . 15, that the first cloned transgenic pigs born. Through the pig DNA ( deoxyribonucleic acid ) for analysis, the body containing the jellyfish gene , and thus is recognized as a transgenic pig . "GM is a bio-technology, is to an exogenous gene into an organism , expressed it in this body , we need to get the gene product ( protein ) ." Between organisms are able to carry out any genetically modified in transgenic animals can also be genetically modified again . Generally, after several transgenic nor artificially " create" a new species ; since species not by a few genes of the decision. However, if too much exogenous genes transferred , may cause the animal damage normal physiological activity , can not survive or reproduce and lead to failure.
Many organisms can be light, but the involvement of protein and luminescence mechanism. Transferred to the pigs in vivo gene contains jellyfish green fluorescent protein , this protein can be excited in the UV light . In Japan cloned transgenic pigs in this experiment , some parts of the pig in the ultraviolet irradiation, emit fluorescence . This is the result of in vivo gene containing the jellyfish . Why jellyfish gene to produce this wonderful phenomenon can ?
" Jellyfish light reason , because it contains a green fluorescent protein of this protein in the UV irradiation, in the  chromium oxide excited state , can absorb photons from the UV- release , emit longer wavelength light , the green . "
There are many kinds of organisms can be light, such as certain bacteria, insects, cnidarians , they can glow with a protein related , but the mechanisms involved are not the same proteins and luminous . For example, fireflies glow of its in vivo oxidation of luciferin luciferase result . In the middle there is a jellyfish called luminous jellyfish protein , calcium ions in the environment is able to issue a blue jellyfish green fluorescent protein in the absorption of the light emitted by jellyfish issued only after the green. "But it was found that the emission of green fluorescent protein does not require the involvement of other factors , as long as irradiation with ultraviolet light can be so , and the green fluorescent protein is extremely stable, and no toxic , so the application of the most biological studies . "

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